B.A.D Index du Forum
Home - FAQ - Rechercher - Membres - Groupes - S’enregistrer - Profil - Se connecter pour vérifier ses messages privés - Connexion
 
C?? For Essential 8

B.A.D Index du Forum -> partie public -> taverne
Poster un nouveau sujet Répondre au sujet Sujet précédent :: Sujet suivant
Auteur Message
arnemm


Hors ligne

Inscrit le: 13 Avr 2016
Messages: 106
Localisation: Montpellier

MessagePosté le: Sam 21 Mai - 01:55 (2016) Sujet du message: C?? For Essential 8 Répondre en citant




C?? For Essential 8 > bit.ly/1U6aNBi





If,,,you,,,continue,,,without,,,changing,,,your,,,settings,,,,we'll,,,assume,,,that,,,you,,,are,,,happy,,,to,,,receive,,,all,,,cookies,,,on,,,Thermo,,,Fisher,,,Scientific,,,websitesOr,,,,continue,,,as,,,a,,,guest,,,buyer,,,to,,,shop,,,for,,,other,,,products,,,Sign,,,in,,,Invalid,,,username,,,and/or,,,passwordCD2650),,,37C,,,water,,,bath,,,Appropriate,,,tissue,,,culture,,,plates,,,and,,,supplies,,,TOP,,,Preparing,,,Media,,,and,,,Materials,,,Essential,,,8,,,Medium,,,(500,,,mL,,,of,,,complete,,,medium),,,Thaw,,,Essential,,,8,,,Supplement,,,(50X),,,at,,,room,,,temperature,,,for,,,1,,,hrHowever,,you,can,change,your,cookie,settings,at,any,time,at,the,bottom,of,this,pageAdd,an,appropriate,volume,of,pre-warmed,Essential,8,Medium,to,each,well,of,a,coated,6-well,plate,so,that,each,well,contains,2,mL,medium,after,the,cell,suspension,has,been,addedIncubate,,,the,,,dishes,,,in,,,a,,,37C,,,,5%,,,CO2,,,incubator,,,for,,,1,,,hourPre-warm,,the,,required,,volume,,of,,Essential,,8,,Medium,,at,,room,,temperature,,until,,it,,is,,no,,longer,,cool,,to,,the,,touchSandwich,Isls.Saint,Kitts,and,NevisSaint,LuciaSaint,Vincent,and,the,GrenadinesSan,MarinoSao,Tome,and,PrincipeSaudi,ArabiaSenegalSerbiaSeychellesSierra,LeoneSingaporeSlovak,RepublicSloveniaSomaliaSouth,AfricaSpainSri,LankaStContinue,Shopping,Close,Session,Aspirate,,the,,diluted,,Geltrex,,solution,,from,,the,,culture,,dish,,and,,discardTOP,Appendix,AResent,,,the,,,verification,,,link,,,Remember,,,Me?,,,Forgot,,,password?,,,Learn,,,more,,,about,,,this,,,Not,,,a,,,member,,,yet?,,,SIGN,,,UP,,,Help,,,Account,,,SIGN,,,IN,,,Not,,,a,,,member,,,yet?,,,SIGN,,,UP,,,Help,,,Dispense,aliquots,of,the,suspension,into,cryogenic,vials,according,to,manufacturers,specifications,(i.e.,,1.5,mL,in,a,2-mL,cryovial)Note:,An,optimal,dilution,of,the,Geltrex,solution,may,need,to,be,determined,for,each,cell,lineCells,,are,,typically,,passaged,,approximately,,24,,hours,,sooner,,than,,they,,would,,be,,in,,other,,feeder-free,,media,,,with,,passaging,,occurring,,when,,the,,cells,,are,,85%,,confluentThats,,why,,,whether,,youre,,running,,acontent,,syndication,,program,,or,,a,,sponsored,,emaillist,,,its,,crucial,,to,,know,,how,,that,,content,,is,,performingOriginally,,developed,,by,,Chen,,et,,al.1,,in,,the,,laboratory,,of,,James,,Thomson,,,and,,validated,,by,,Cellular,,Dynamics,,International,,,Essential,,8,,Medium,,has,,been,,extensively,,tested,,and,,is,,proven,,to,,maintain,,pluripotency,,in,,multiple,,PSC,,linesAspirate,residual,vitronectin,solution,from,the,pre-coated,dishAliquot,1,mL,of,diluted,vitronectin,solution,to,each,well,of,a,6-well,plate,(refer,to,Table,1,for,recommended,volumes,for,other,culture,vessels)Your,company,will,need,to,define,its,own,acceptable,range,of,ratios,,indicating,which,investments,you,should,repeat,,and,which,should,be,either,avoided,or,adjustedFigure,1,AThis,,also,,tells,,you,,which,,content,,is,,driving,,prospects,,to,,become,,customers,,,which,,will,,help,,you,,plan,,your,,next,,round,,of,,content,,assetsNote:,Before,use,,warm,complete,medium,required,for,that,day,at,room,temperature,until,it,is,no,longer,cool,to,the,touchnoA1517001),,Vitronectin,,,truncated,,recombinant,,human,,(VTN-N),,(Cat.If,,you,,continue,,without,,changing,,your,,settings,,,we'll,,assume,,that,,you,,are,,happy,,to,,receive,,all,,cookies,,on,,Thermo,,Fisher,,Scientific,,websitesHelenaSurinameSvalbard,and,Jan,Mayen,IslandsSwazilandSwedenSwitzerlandSyriaTaiwan,,TajikistanTanzaniaThailandTogoTongaTrinidad,and,TobagoTunisiaTurkeyTurkmenistanTurks,and,Caicos,IslandsUgandaUkraineUnited,Arab,EmiratesUnited,KingdomUnited,StatesUnited,States,Minor,Outlying,IslandsUruguayUzbekistanVatican,City,State,(Holy,See)VenezuelaViet,NamVirgin,Islands,(British)Virgin,Islands,(U.S.)Western,SaharaYemenZambiaZimbabwe,Go,Cancel,*,To,learn,more,about,enabling,cookies,,visit,our,Internet,Settings,pageIn,,addition,,,Essential,,8™,,Medium,,has,,been,,used,,to,,scale,,up,,production,,of,,iPSCs,,and,,shown,,to,,support,,iPSC,,growth,,for,,>50,,passages,,without,,any,,signs,,of,,karyotypic,,abnormalities,,,along,,with,,maintaining,,the,,ability,,of,,iPSCs,,to,,differentiate,,into,,all,,three,,germ,,line,,lineages.Features,,of,,Essential,,8™,,Medium,,include:•,,Consistent—reduced,,variability,,compared,,to,,existing,,feeder-free,,culture,,media•,,Robust—reliable,,and,,robust,,cultures,,with,,a,,xeno-free,,,cGMP,,,8-component,,medium•,,Cost,,effective—economical,,and,,scalable,,PSC,,culture,,compared,,to,,other,,feeder-free,,mediaReduced,,VariabilityEssential,,8™,,Medium,,is,,xeno-free,,and,,contains,,only,,the,,8,,essential,,components,,needed,,for,,stem,,cell,,cultureSerum-Free,,,&,,,Specialty,,,Media›,,,Stem,,,Cell,,,Media›,,,Essential,,,8™,,,Medium,,,Please,,,purchase,,,this,,,product,,,by,,,removing,,,it,,,from,,,your,,,on-site,,,supply,,,centerAdd,,,PSC,,,Cryomedium,,,(chilled,,,to,,,2C,,,to,,,8,,,C),,,dropwise,,,to,,,the,,,cells,,,while,,,gently,,,rocking,,,the,,,tube,,,back,,,and,,,forth,,,followed,,,by,,,gentle,,,resuspension,,,of,,,cell,,,pellet15575-020),,,Dulbeccos,,,Modified,,,Eagle,,,Medium,,,(DMEM),,,with,,,GlutaMAX-I,,,(Cat(Optional):,,To,,improve,,cell,,survival,,,you,,can,,add,,RevitaCell,,Supplement,,(CatContact,Us,Account,Sign,In,Register,Account,Orders,Favorite,Lists,Custom,Products,&,Projects,Instrument,Management,Manage,instrument,use,,information,,and,service,Profile,Quick,Order,0,Ship,To,Lab,Supply,Center,Order,Supply,Centre,Order,Go,Applications,&,TechniquesMost,Popular,Products,Lab,SolutionsLab,AutomationLab,Data,Management,&,Analysis,SoftwareLab,EquipmentLab,Plasticware,&,SuppliesBrandsThermo,ScientificApplied,BiosystemsInvitrogenGibcoIon,TorrentLife,SciencesAntibodiesCell,AnalysisCell,Culture,&,TransfectionCloning,,Synthetic,BiologyDNA,&,RNA,PurificationEpigeneticsFlow,CytometryGene,Expression,AnalysisGenome,EditingPCR,,Real-time,PCRProtein,BiologyRNAiSequencingStem,Cell,ResearchIndustrial,&,Applied,ScienceAgricultural,BiotechnologyAnimal,HealthBioproductionCement,,Coal,&,MineralsChromatographyEnvironmentalFood,&,BeverageForensicsManufacturing,&,ProcessingMass,SpectrometryMicrobiologyPharma,&,BiopharmaRadiation,Detection,&,MeasurementSpectroscopy,,Elemental,&,Isotope,AnalysisClinical,&,DiagnosticsAnatomical,PathologyCancer,ResearchClinical,MicrobiologyClinical,&,Translational,ResearchDiagnostic,DevelopmentDiagnostic,TestingPreclinical,to,Companion,Diagnostic,DevelopmentPublic,HealthProduct,Selection,GuidesServicesNew,ProductsPromotionsLearning,CentersShop,All,Products,Most,Popular,ProductsBeakersLab,CentrifugesLab,Ovens,&,FurnacesLipofectamine,ReagentsNalgene,LabwareOligos,,Primers,,Probes,&,NucleotidesPipettes,&,Pipette,TipsSuperScript,Reverse,TranscriptaseTaqMan,Real-Time,PCR,AssaysTRIzol,ReagentsMost,Popular,CategoriesAntibodies,&,ImmunoassaysCell,Culture,&,Transfection,ReagentsChromatography,Columns,,Resins,,&,Spin,FiltersDrug,Discovery,AssaysGels,&,MembranesInstruments,&,EquipmentLab,Reagents,&,ChemicalsLab,Supplies,,Plastics,&,GlasswarePrimers/Oligos,,Cloning,&,Gene,SynthesisSoftware,&,Digital,StorageOrder,ToolsHow,to,OrderQuick,OrderTrack,Your,OrderOrder,HistoryOrder,SupportContact,UsShop,All,ProductsShop,All,ServicesProduct,Selection,GuidesServices,&,SupportProduct,DocumentationCertificates,of,Analysis,and,ConformanceChemical,StructuresCitations,&,ReferencesManufacturing,Site,ISO,CertificationsManualsMedia,FormulationsNewsletters,&,JournalsProduct,LiteratureRoHS,CertificatesSDSsSpectral,DataSupplemental,ProtocolsVector,MapsServicesCustom,ServicesEnterprise-Level,Lab,InformaticsEnterprise,ServicesFinancial,and,Leasing,ServicesInstrument,ServicesLicensing,and,Commercial,SupplyTraining,&,EducationEventsHow,To,&,Educational,VideosLearning,CentersTraining,ServicesWebinarsProduct,SupportInstrument,SupportKnowledge,Base/Product,FAQsLife,Sciences,Support,CentersContact,UsTools,&,CalculatorsAntibody,SearchBiotinylation,Reagent,Selection,ToolCell,Staining,ToolCrosslinker,Selection,ToolELISA,Kits,by,Target,ToolFluorescence,SpectraViewerLab,AppsRestriction,Enzyme,FinderSpectra,DataThermo,Scientific,Molecular,Biology,ToolsVector,Selection,ToolAbout,Us,EventsAbout,Us,Careers,Investors,News,Responsibility,Cloud,Note:,You,clicked,on,an,external,link,,which,has,been,disabled,in,order,to,keep,your,shopping,session,openAdd,,1X,,Versene,,solution,,to,,the,,vessel,,containing,,PSCs f5880b0734



Revenir en haut

Auteur Message
Publicité






MessagePosté le: Sam 21 Mai - 01:55 (2016) Sujet du message: Publicité

PublicitéSupprimer les publicités ?
Revenir en haut

Poster un nouveau sujet Répondre au sujet B.A.D Index du Forum -> partie public -> taverne Toutes les heures sont au format GMT + 1 Heure
Page 1 sur 1


Sauter vers:  

Index | créer un forum | Forum gratuit d’entraide | Annuaire des forums gratuits | Signaler une violation | Conditions générales d'utilisation
Powered by phpBB © 2001,2003 phpBB GroupTraduction par : phpBB-fr.com. Black_Xire logo © 2004 BaRiMzI. All images except original subSilver images are property/trademarks of RiMzInK.com. Black_Xire made by BaRiMzI